解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::High affinity aptamer-based biomarker discovery has the advantage of simultaneously discovering an aptamer affinity reagent and its target biomarker protein. Here, we demonstrate a morphology-based tissue aptamer selection method that enables us to use tissue sections from individual patients and identify high-affinit...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114473
更新日期:2016-11-01 00:00:00
abstract::GC-rich DNA regions were PCR-amplified with Taq DNA polymerase using either the canonical set of deoxynucleoside triphosphates or mixtures in which the dCTP had been partially or completely replaced by its N4-methylated analog, N4-methyl-2'-deoxycytidine 5'-triphosphate (N4me-dCTP). In the case of a particularly GC-ri...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114457
更新日期:2016-10-01 00:00:00
abstract::Address correspondence to Mart Speek, Department of Gene Technology, Akadeemia tee 15, Room 129, Tallinn University of Technology, Tallinn 19086, Estonia. E-mail: mart.speek@ttu.ee. ...
journal_title:BioTechniques
pub_type: 信件
doi:10.2144/000114441
更新日期:2016-08-01 00:00:00
abstract::Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes-SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, Eva...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114432
更新日期:2016-07-01 00:00:00
abstract::Single-cell genomics (SCG) is a recently developed tool to study the genomes of unculturable bacterial species. SCG relies on multiple-strand displacement amplification (MDA), PCR, and next-generation sequencing (NGS); however, obtaining sufficient amounts of high-quality DNA from samples is a major challenge when per...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114389
更新日期:2016-03-01 00:00:00
abstract::Caspase-3 is a key effector caspase that is activated in both extrinsic and intrinsic pathways of apoptosis. Available fluorescent sensors for caspase-3 activity operate in relatively short wavelength regions and are nonoptimal for multiparameter microscopy and whole-body imaging. In the present work, we developed new...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114377
更新日期:2016-02-01 00:00:00
abstract::Extracellular vesicles (EVs) are spherical membrane structures released by most cells. These highly conserved mediators of intercellular communication carry proteins, lipids, and nucleic acids, and transfer these cellular components between cells by different mechanisms, such as endocytosis, macropinocytosis, or fusio...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114371
更新日期:2016-01-01 00:00:00
abstract::Here we report a simple new method for exposing cells to normoxic and hypoxic conditions using vacuum bags, normally employed for food storage, to establish and maintain low oxygen levels in vitro. Vacuum bags were gassed with a mixture containing specified levels of oxygen, then sealed, creating a hypoxic microenviro...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114341
更新日期:2015-10-01 00:00:00
abstract::Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene synthesis or decrease variability when applied to the constructi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114330
更新日期:2015-09-01 00:00:00
abstract::DNA damage in the form of abasic sites, chemically altered nucleotides, and strand fragmentation is the foremost limitation in obtaining genetic information from many ancient samples. Upon cell death, DNA continues to endure various chemical attacks such as hydrolysis and oxidation, but repair pathways found in vivo n...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114307
更新日期:2015-07-01 00:00:00
abstract::Ribosome profiling is a powerful tool for characterizing in vivo protein translation at the genome scale, with multiple applications ranging from detailed molecular mechanisms to systems-level predictive modeling. Though highly effective, this intricate technique has yet to become widely used in the microbial research...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114302
更新日期:2015-06-01 00:00:00
abstract::Cysts of free-living protozoa have an impact on the ecology and epidemiology of bacteria because they may act as a transmission vector or shelter the bacteria against hash environmental conditions. Detection and localization of intracystic bacteria and examination of the en- and excystment dynamics is a major challeng...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114274
更新日期:2015-04-01 00:00:00
abstract::The Drosophila heart has gained considerable traction as a model of cardiac development and physiology. Previously we described a semiautomatic optical heartbeat analysis (SOHA) method for quantifying functional parameters from the fly heart that facilitated its use as an organ system and disease model. Here we presen...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114255
更新日期:2015-02-01 00:00:00
abstract::Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all Escherichia coli cell-free expression system. Our met...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114249
更新日期:2015-01-01 00:00:00
abstract::The high-order structure of human chromosomes is an important biological question that is still under investigation. Studies have been done on imaging human mitotic chromosomes using mostly 2-D microscopy methods. To image micron-sized human chromosomes in 3-D, we developed a procedure for preparing samples for serial...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114236
更新日期:2014-12-01 00:00:00
abstract::Next-generation sequencing (NGS) of multigene panels performed for genetic clinical diagnostics requires 100% coverage of all targeted genes. In the genetic diagnostics laboratory, coverage gaps are typically filled with Sanger sequencing after NGS data are collected and analyzed. Libraries prepared using the hybridiz...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114217
更新日期:2014-10-01 00:00:00
abstract::A method for conserving primers and differently labeled fluorogenic hydrolysis (i.e., TaqMan) probes at ambient conditions is presented. Primers and hydrolysis probes with four different fluorophore-quencher combinations (6- FAM-BHQ1, HEX-BHQ1, ROX-BHQ650, and Cy5-BHQ2) were mixed with trehalose and xanthan at final c...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114207
更新日期:2014-09-01 00:00:00
abstract::In the quest to move more basic research discoveries into the clinic, the medical community is building training programs to help researchers gain "translational" skills. Sarah Webb explores how PhDs are learning to speak the language of the clinic. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/000114194
更新日期:2014-08-01 00:00:00
abstract::Thin spun-coat films (~4 nm thick) of graphene oxide (GO) constitute a versatile surface chemistry compatible with a broad range of technologically important sensor materials. Countless publications are dedicated to the nuances of surface chemistries that have been developed for sensors, with almost every material hav...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114188
更新日期:2014-07-01 00:00:00
abstract::Recent computational and bioinformatics advances have enabled the efficient creation of novel biocatalysts by reducing amino acid variability at hot spot regions. To further expand the utility of this strategy, we present here a tool called Multi-site Degenerate Codon Analyzer (MDC-Analyzer) for the automated design o...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114177
更新日期:2014-06-01 00:00:00
abstract::Current protocols for screening proliferative factors for β cells ex vivo are time-consuming, require cell lines or dissociated islets, and often entail expensive specialized screening equipment. Here we present an efficient and lower cost alternative that utilizes intact mouse islets for the initial screening of prol...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114115
更新日期:2013-12-01 00:00:00
abstract::Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Her...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114104
更新日期:2013-11-01 00:00:00
abstract::Comprehensive analysis of DNA methylation patterns is critical for understanding the molecular basis of many human diseases. While hundreds of PCR-based DNA methylation studies are published every year, the selection and implementation of appropriate methods for these studies can be challenging for molecular genetics ...
journal_title:BioTechniques
pub_type: 杂志文章,评审
doi:10.2144/000114087
更新日期:2013-10-01 00:00:00
abstract::Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material. We describe a new technique, polymerase chain displacement reaction (PCDR), which uses multiple nested primers in...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113951
更新日期:2013-02-01 00:00:00
abstract::prfectBLAST is a multiplatform graphical user interface (GUI) for the stand-alone BLAST+ suite of applications. It allows researchers to do nucleotide or amino acid sequence similarity searches against public (or user-customized) databases that are locally stored. It does not require any dependencies or installation a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113953
更新日期:2012-11-01 00:00:00
abstract::Fluorescent dye terminator Sanger sequencing (FTSS), with detection by automated capillary electrophoresis (CE), has long been regarded as the gold standard for variant detection. However, software analysis and base-calling algorithms used to detect mutations were largely optimized for resequencing applications in whi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113913
更新日期:2012-09-01 00:00:00
abstract::Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg wh...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113914
更新日期:2012-08-01 00:00:00
abstract::Loop-mediated isothermal amplification (LAMP) is a rapid and reliable sequence-specific isothermal nucleic acid amplification technique. To date, all reported real-time detection methods for LAMP have been restricted to single targets, limiting the utility of this technique. Here, we adapted standard LAMP primers to c...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/0000113902
更新日期:2012-08-01 00:00:00
abstract::Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproducibly accurate resul...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/0000113854
更新日期:2012-07-01 00:00:00
abstract::Microarray platforms require analytical pipelines with modules for data pre-processing including data normalization, statistical analysis for identification of differentially expressed genes, cluster analysis, and functional annotation. We previously developed the Automated Microarray Data Analysis (AMDA, version 2.3....
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/0000113889
更新日期:2012-07-01 00:00:00
abstract::The relative spatial distribution of cells in a solid tumor contributes to development of malignancy, yet the details of this process remain poorly understood. To elucidate these mechanisms, the ability to extract and analyze the entire DNA content of individual cells whose precise location in the tumor is known is re...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113860
更新日期:2012-04-01 00:00:00
abstract::We observed the presence of contaminating NADH oxidation activity in maltose binding protein (MBP) fusion proteins expressed in Escherichia coli and purified using conventional amylose resin-based affinity chromatography. This contaminating NADH oxidation activity was detectable with at least four different enzymes fr...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/0000113822
更新日期:2012-04-01 00:00:00
abstract::The nematode Caenorhabditis elegans is an important model animal for biological research. Currently, transgenic C. elegans strains are mainly generated by injecting DNA encoding a gene of interest, in combination with a reporter gene, into the gonad. With this approach, the interpretation of negative results, such as ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113821
更新日期:2012-03-01 00:00:00
abstract::Tissue engineering scaffolds have emerged as a powerful tool within regenerative medicine. These materials are being designed to create environments that promote regeneration through a combination of: (i) scaffold architecture, (ii) the use of scaffolds as vehicles for transplanting progenitor cells, and/or (iii) loca...
journal_title:BioTechniques
pub_type: 杂志文章,评审
doi:10.2144/000113754
更新日期:2011-10-01 00:00:00
abstract::Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113738
更新日期:2011-09-01 00:00:00
abstract::We have designed and built a magnetic tweezers device that enables the application of calibrated stresses to soft materials while simultaneously measuring their microscale deformation using confocal microscopy. Unlike previous magnetic tweezers designs, our device is entirely portable, allowing easy use on microscopes...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113701
更新日期:2011-07-01 00:00:00
abstract::The type IIS/IIC restriction endonuclease TspGWI recognizes the sequence 5'-ACGGA-3', cleaving DNA 11/9 nucleotides downstream. Here we show that sinefungin, a cofactor analog of S-adenosyl methionine, induces a unique type of relaxation in DNA recognition specificity. In the presence of sinefungin, TspGWI recognizes ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113685
更新日期:2011-06-01 00:00:00
abstract::We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0-4°C permits modification of the protease method of surface analysis to temperatures at which trafficking of mammalian plasmalemmal proteins is blocked. This is an imp...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113651
更新日期:2011-04-01 00:00:00
abstract::Three-dimensional collagen gel contraction is the standard assay utilized for functionally quantifying a variety of cell types, in particular smooth muscle cells (SMCs) and myofibroblasts. Here, we have developed a method to effectively reduce the three-dimensional parameters of the standard collagen gel into a single...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113349
更新日期:2010-02-01 00:00:00
abstract::The study of gene regulation in cardiac myocytes requires a reliable in vitro model. However, monolayer cultures used for this purpose are typically not exposed to electrical stimulation, though this has been shown to strongly affect cardiomyocyte gene expression. Based on pacemakers for clinical use, we developed an ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113308
更新日期:2010-01-01 00:00:00